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Amaxa nucleofector device
Nucleofector Device, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nucleofector+device/device+nucleofector/pmc13135601-26-36-38
Average 86 stars, based on 1 article reviews
nucleofector device - by Bioz Stars, 2026-10
86/100 stars

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Knockdown:

Article Title: Starvation of leukemic cells enhances DNA damage-induced apoptosis in vitro via ROS/p38 MAPK and prevents leukemia progression in fasting xenograft mice.
Article Snippet: .. Knockdown of proteins by transfection with small-interfering RNA oligonucleotides REH cells (4x106) were transfected with small interfering RNA (siRNA) using a nucleofector device (Amaxa Biosciences) and the Nucleofector® Kit R (Lonza) according to the manufacturer’s instructions and using the program G-009 as previously described (10). ..

Transfection:

Article Title: Starvation of leukemic cells enhances DNA damage-induced apoptosis in vitro via ROS/p38 MAPK and prevents leukemia progression in fasting xenograft mice.
Article Snippet: .. Knockdown of proteins by transfection with small-interfering RNA oligonucleotides REH cells (4x106) were transfected with small interfering RNA (siRNA) using a nucleofector device (Amaxa Biosciences) and the Nucleofector® Kit R (Lonza) according to the manufacturer’s instructions and using the program G-009 as previously described (10). ..

Article Title: Therapeutic Induction of Ferroptosis in Tumors Using PD-L1 Targeting Antibody Nanogel Conjugates
Article Snippet: .. The RNP complexes were transfected in cells using Nucleofector Device (Amaxa). ..

Article Title: Starvation of leukemic cells enhances DNA damage-induced apoptosis in vitro via ROS/p38 MAPK and prevents leukemia progression in fasting xenograft mice
Article Snippet: .. REH cells (4 × 10 6 ) were transfected with small interfering RNA (siRNA) using a nucleofector device (Amaxa Biosciences) and the Nucleofector® Kit R (Lonza) according to the manufacturer’s instructions and using the program G-009 as previously described ( ). ..

Small Interfering RNA:

Article Title: Starvation of leukemic cells enhances DNA damage-induced apoptosis in vitro via ROS/p38 MAPK and prevents leukemia progression in fasting xenograft mice.
Article Snippet: .. Knockdown of proteins by transfection with small-interfering RNA oligonucleotides REH cells (4x106) were transfected with small interfering RNA (siRNA) using a nucleofector device (Amaxa Biosciences) and the Nucleofector® Kit R (Lonza) according to the manufacturer’s instructions and using the program G-009 as previously described (10). ..

Article Title: Starvation of leukemic cells enhances DNA damage-induced apoptosis in vitro via ROS/p38 MAPK and prevents leukemia progression in fasting xenograft mice
Article Snippet: .. REH cells (4 × 10 6 ) were transfected with small interfering RNA (siRNA) using a nucleofector device (Amaxa Biosciences) and the Nucleofector® Kit R (Lonza) according to the manufacturer’s instructions and using the program G-009 as previously described ( ). ..

Allele-specific Oligonucleotide:

Article Title: A nonenzymatic dependency on inositol-requiring enzyme 1 controls cancer cell cycle progression and tumor growth
Article Snippet: .. 1 × 10 6 cells were electroporated with 10 μM of NTC ASO (5′-GACTATACGCGCAATA-3′) or IRE targeting ASO (5′-AATTGCCGGTCCTTCT-3′), using nucleofector reagent cocktail (Lonza), cuvettes (Lonza) and the nucleofector device (Amaxa) in the “HCT116” function. ..

Article Title: A nonenzymatic dependency on inositol-requiring enzyme 1 controls cancer cell cycle progression and tumor growth.
Article Snippet: .. 1 × 106 cells were electroporated with 10 μM of NTC ASO (5′-GACTATACGCGCAATA-3′) or IRE targeting ASO (5′-AATTGCCGGTCCTTCT-3′), using nucleofector reagent cocktail (Lonza), cuvettes (Lonza) and the nucleofector device (Amaxa) in the “HCT116” function. ..

other:

Article Title: High-throughput screen of 100 000 small molecules in C9ORF72 ALS neurons identifies spliceosome modulators that mobilize G4C2 repeat RNA into nuclear export and repeat associated non-canonical translation
Article Snippet: Approx 1 × 10 6 iPS cells were nucleofected by Amaxa nuclefector device using Human Stem Cell Nucleofector® Kit 1 (Lonza #VPH-5012) and Prg#B-016 with 4 ug of Ngn2 plasmid and 1 ug of the dual helper plasmid.

Plasmid Preparation:

Article Title: Estradiol alters actin and protrusion dynamics in endometriotic epithelial cells
Article Snippet: .. Briefly, cells were suspended in 100μL of a supplemented nucleofector solution (Lonza, Cell Line Nucleofector Kit L, cat. #VCA-1005, Germany) with 5 μg of LifeAct-GFP plasmid, transferred to a cuvette, and subjected to electroporation via the Nucleofector device (Amaxa, Germany). ..

Article Title: Per- and Polyfluoroalkyl Substances (PFAS) Enhance Cholesterol Accumulation and Dysregulate Inflammatory Responses in Macrophages
Article Snippet: .. 1 × 106 cells were resuspended in 100 μL of Nucleofector Solution V and electroporated with 1.0 μg of plasmid DNA using program V-001 on the Nucleofector device (Amaxa GmbH, Köln, Germany). ..

Electroporation:

Article Title: Estradiol alters actin and protrusion dynamics in endometriotic epithelial cells
Article Snippet: .. Briefly, cells were suspended in 100μL of a supplemented nucleofector solution (Lonza, Cell Line Nucleofector Kit L, cat. #VCA-1005, Germany) with 5 μg of LifeAct-GFP plasmid, transferred to a cuvette, and subjected to electroporation via the Nucleofector device (Amaxa, Germany). ..



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